Clement E. Furlong
Departments of Medicine (Div. Medical Genetics) & Genome Sciences, University of Washington
- Professor at the University of Washington.
- Ph.D. Biochemistry, University of California, Davis, 1968; BA Chemistry, San Jose State College, 1963
- Postdoctoral fellowship, Biochemistry and Molecular Biology, Cornell University, Ithaca, 1968-1970.
- Sabbatical, Immunology, Stanford University, 1976-1977, Harden McConnell, Hugh McDevitt mentors.
Dr. Furlong's laboratory works on the development of portable surface plasmon resonance (SPR) biosensors that have a broad range of applications in the fields of medicine, biotechnology and environmental monitoring. For monitoring applications, the sensors are capable of detecting small molecules such as hormones, insecticides, nerve agents as well as larger analytes including toxic proteins, viruses and microbes. As general laboratory instruments, the SPR biosensor systems are capable of monitoring in real time, biomolecular interactions such as the interaction of transcription factors with specific DNA sequences, interactions of protein subunits with complexes, receptor/ligand interactions and rapid protein detection capable of automating on-line protein fractionation.
The laboratory also works on protein engineering and expression in E. coli systems.
Dr. Furlong's laboratory is also investigating the functional genomics of the polymorphic, HDL-associated enzyme human serum paraoxonase (PON1). The main known physiological function of this protein is to metabolize toxic oxidized lipids and protect against vascular disease. Low levels of PON1 or mutations in PON1 are associated with a number of diseases.
Portable surface plasmon resonance (SPR) biosensors, protein engineering, protein purification, genetic variability of susceptibility to insecticide exposure and risk of vascular disease.